Removal of the spleen, the largest single lymphoid organ and the second largest organ of the reticuloendothelial system. Leads to increase in severe infection or overwhelming sepsis. Splenectomized individuals. Have many immunological
abnormalities. To
evaluate the alteration of lymphocyte subsets of the peripheral blood in the splenectomized patients, we have examined lymphocyte and its subsets chonges serially during the postoperative 3rd day and 7th day, and 14th in 15 splenectomized
patients
due
to trauma(10 patients) and hypersplenism(5 patients), as well as 10 cases of control group who underwent other abdominal surgery.
The data were presented in absolute counts using FACScan cytometer.
@ES In comparison with control group.
@EN 1) Splenectomized patients due to trauma(trauma group) showed decreased levels of total lymphocyte and T lymphocyte(CD3) and helper inducer T lymphocyte(CD4, TH) and suppressor/cytotoxic T lymphocyte(CD8. Ts) on 3rd day after operation. These
differences were statistically significant(p<0.01). But on 7th and 14th day. These values was significantly increased or tended to be increased than that of control group.
2) In splenectomized patients due to hypersplenism(hypersplenism group), the absolute number of the total lymphocyte and its subsets(CD3, CD4, CD8) level were significantly decreased to about one-half of control group on postoperative 7th and
14th
day(p<0.05).
3) The change of CD4/CD8 ratio revealed no statistical difference during this period in both splenectomized and control group.
4) In both groups of the splenectomized patients, the changes of B lymphocyte and NK cell were revealed no significant statistical difference until 14th day after operation, but which were tended to be decreased than that of control group.
On the basis of this results, it can be suggested that the phenotypic changes of lymphocyte and its subsets after splenectomy were mainly influenced by T lymphocyte than B lymphocyte.
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